Application of the Mars Organic Analyzer to nucleobase and amine biomarker detection

The Mars Organic Analyzer (MOA), a portable microfabricated capillary electrophoresis instrument being developed for planetary exploration, is used to analyze a wide variety of fluorescamine-labeled amine-containing biomarker compounds, including amino acids, mono and diaminoalkanes, amino sugars, nucleobases, and nucleobase degradation products. The nucleobases cytosine and adenine, which contain an exocyclic primary amine, were effectively labeled, separated, and detected at concentrations <500 nM. To test the general applicability of the MOA for biomarker detection, amino acids and mono- and diamines were extracted from bacterial cells using both hydrolysis and sublimation followed by analysis. The extrapolated limit of detection provided by the valine biomarker was approximately 4 x 10(3) cells per sample. Products of an NH(4)CN polymerization that simulate a prebiotic synthesis were also successfully isolated via sublimation and analyzed. Adenine and alanine/serine were detected with no additional sample cleanup at 120 +/- 13 microM and 4.1 +/- 1 microM, respectively, corresponding to a reaction yield of 0.04% and 0.0003%, respectively. This study demonstrates that the MOA provides sensitive detection and analysis of low levels of a wide variety of amine-containing organic compounds from both biological and abiotic sources.     

灰色系统模型研究进展(英文)

针对灰色系统模型的研究进展进行了综述,介绍了一般灰数的概念、灰色序列算子和一些常用的灰色系统模型,包括灰色绝对关联度模型、灰色相对关联度模型、基于端点三角白化权函数的灰色聚类评估模型、基于中心点三角白化权函数的灰色聚类评估模型、GM(1,1)预测模型以及多目标加权灰靶决策模型。     

Design and Performance of the ICON EUV Spectrograph

We present the design, implementation, and on-ground performance measurements of the Ionospheric Connection Explorer EUV spectrometer, ICON EUV, a wide field (\(17^{\circ}\times 12^{\circ}\)) extreme ultraviolet (EUV) imaging spectrograph designed to observe the lower ionosphere at tangent altitudes between 100 and 500 km. The primary targets of the spectrometer, which has a spectral range of 54–88 nm, are the Oii emission lines at 61.6 nm and 83.4 nm. Its design, using a single optical element, permits a $0\stackrel{{}^{°}}{.}26$ imaging resolution perpendicular to the spectral dispersion direction with a large (\(12^{\circ} \)) acceptance parallel to the dispersion direction while providing a slit-width dominated spectral resolution of \(R\sim25\) at 58.4 nm. Pre-flight calibration shows that the instrument has met all of the science performance requirements.     

GHRS observations of the LISM

The GHRS has obtained high-resolution spectra of interstellar gas toward 19 nearby stars. These excellent data show that the Sun is located inside the Local Interstellar Cloud (LIC) with other warm clouds nearby. I will summarize the physical properties of these clouds and the three-dimensional structure of this warm interstellar gas. There is now clear evidence that the Sun and other late-type stars are surrounded by hydrogen walls in the upwind direction. The D/H ratio probably has a constant value in the LIC, (1.6 ± 0.2) × 10^–5, consistent with the measured values for all LIC lines of sight.     

Exposure of Arabidopsis thaliana to hypobaric environments: implications for low-pressure bioregenerative life support systems for human exploration missions and terraforming on Mars

Understanding how hypobaria can affect net photosynthetic (P (net)) and net evapotranspiration rates of plants is important for the Mars Exploration Program because low-pressured environments may be used to reduce the equivalent system mass of near-term plant biology experiments on landers or future bioregenerative advanced life support systems. Furthermore, introductions of plants to the surface of a partially terraformed Mars will be constrained by the limits of sustainable growth and reproduction of plants to hypobaric conditions. To explore the effects of hypobaria on plant physiology, a low-pressure growth chamber (LPGC) was constructed that maintained hypobaric environments capable of supporting short-term plant physiological studies. Experiments were conducted on Arabidopsis thaliana maintained in the LPGC with total atmospheric pressures set at 101 (Earth sea-level control), 75, 50, 25 or 10 kPa. Plants were grown in a separate incubator at 101 kPa for 6 weeks, transferred to the LPGC, and acclimated to low-pressure atmospheres for either 1 or 16 h. After 1 or 16 h of acclimation, CO(2) levels were allowed to drawdown from 0.1 kPa to CO(2) compensation points to assess P (net) rates under different hypobaric conditions. Results showed that P (net) increased as the pressures decreased from 101 to 10 kPa when CO(2) partial pressure (pp) values were below 0.04 kPa (i.e., when ppCO2 was considered limiting). In contrast, when ppCO(2) was in the nonlimiting range from 0.10 to 0.07 kPa, the P (net) rates were insensitive to decreasing pressures. Thus, if CO(2 )concentrations can be kept elevated in hypobaric plant growth modules or on the surface of a partially terraformed Mars, P (net) rates may be relatively unaffected by hypobaria. Results support the conclusions that (i) hypobaric plant growth modules might be operated around 10 kPa without undue inhibition of photosynthesis and (ii) terraforming efforts on Mars might require a surface pressure of at least 10 kPa (100 mb) for normal growth of deployed plant species.     

Middle-atmosphere tides: Recent advances in theory and observation

Recent progress in the theoretical and observational investigation of middle atmosphere tides is reviewed. Theoretical advances include development of more realistic thermal excitation and numerical simulation models, investigation of seasonal — latitudinal and longitudinal variations in tidal structures, and the origin and effects of ‘unsteadiness’ in tidal oscillations over periods of days. Recent analyses of middle latitude radar data between 80 Km and 130 Km delineate consistent seasonal phase patterns in this region. Analyses of rocket data show stratosphere and lower mesosphere tides to be characterized by smaller amplitudes, shorter vertical scales, greater unsteadiness, and greater spatial variability than exhibited in the upper mesosphere and above. Results from the first two ATMAP (Atmospheric Tides Middle Atmosphere Program) campaigns are also discussed.     

Neural network development for the forecasting of upper atmosphere parameter distributions

This paper presents a neural network modeling approach to forecast electron concentration distributions in the 150–600 km altitude range above Arecibo, Puerto Rico. The neural network was trained using incoherent scatter radar data collected at the Arecibo Observatory during the past two decades, as well as the Kp geomagnetic index provided by the National Space Science Data Center. The data set covered nearly two solar cycles, allowing the neural network to model daily, seasonal, and solar cycle variations of upper atmospheric parameter distributions. Two types of neural network architectures, feedforward and Elman recurrent, are used in this study. Topics discussed include the network design, training strategy, data analysis, as well as preliminary testing results of the networks on electron concentration distributions.     

The Energetic Particle and Plasma Spectrometer Instrument on the MESSENGER Spacecraft

The Energetic Particle and Plasma Spectrometer (EPPS) package on the MErcury Surface, Space ENvironment, GEochemistry, and Ranging (MESSENGER) mission to Mercury is composed of two sensors, the Energetic Particle Spectrometer (EPS) and the Fast Imaging Plasma Spectrometer (FIPS). EPS measures the energy, angular, and compositional distributions of the high-energy components of the in situ electrons (>20 keV) and ions (>5 keV/nucleon), while FIPS measures the energy, angular, and compositional distributions of the low-energy components of the ion distributions (<50 eV/charge to 20 keV/charge). Both EPS and FIPS have very small footprints, and their combined mass (∼3 kg) is significantly lower than that of comparable instruments.     

Tírez lake as a terrestrial analog of Europa

Tírez Lake (La Mancha, central Spain) is proposed as a terrestrial analogue of Europa's ocean. The proposal is based on the comparison of the hydrogeochemistry of Tírez Lake with the geochemical features of the alteration mineralogy of meteoritic precursors and with Galileo's Near Infrared Mapping Spectrometer data on Europa's surface. To validate the astrobiological potential of Tírez Lake as an analog of Europa, different hydrogeochemical, mineral, and microbial analyses were performed. Experimental and theoretical modeling helped to understand the crystallization pathways that may occur in Europa's crust. Calculations about the oxidation state of the hypothetical Europan ocean were estimated to support the sulfate-rich neutral liquid model as the origin of Europa's observed hydrated minerals and to facilitate their comparison with Tírez's hydrogeochemistry. Hydrogeochemical and mineralogical analyses showed that Tírez waters corresponded to Mg-Na-SO(4)-Cl brines with epsomite, hexahydrite, and halite as end members. A preliminary microbial ecology characterization identified two different microbial domains: a photosynthetically sustained community represented by planktonic/benthonic forms and microbial mat communities, and a subsurficial anaerobic realm in which chemolithotrophy predominates. Fluorescence in situ hybridization has been used to characterize the prokaryotic diversity of the system. The subsurficial community seemed to be dominated by sulfate-reducing bacteria and methanogens. Frozen Tírez brines were analyzed by Fourier-transform infrared techniques providing spectra similar to those reported previously using pure components and to the Galileo spectral data. Calorimetric measurements of Tírez brines showed pathways and phase metastability for magnesium sulfate and sodium chloride crystallization that may aid in understanding the processes involved in the formation of Europa's icy crust. The use of fluorescence hybridization techniques for microbial detection and characterization in hypersaline environments makes this methodology strongly advisable for future Europa astrobiological missions.